Optimization of Pre-Inoculum, Fermentation Process Parameters and Precursor Supplementation Conditions to Enhance Apigenin Production by a Recombinant Streptomyces albus Strain

Streptomyces albus J1074-pAPI (Streptomyces albus-pAPI) is a recombinant strain constructed to biotechnologically produce apigenin, flavonoid with interesting bioactive features that up now has been manufactured by extraction from plants long and not environmentally friendly procedures. So far, in literature, only maximum apigenin concentration of 80.0 µg·L?1 obtained shake flasks. In this paper, three integrated fermentation strategies were exploited enhance the production J1074-pAPI, combining specific approaches for pre-inoculum conditions, optimization process parameters supplementation precursors. Using mycelium, increased 1.8-fold flask physiological studies. 2L batch fermentation, aeration stirring conditions optimized new inoculum approach reached 184.8 ± 4.0 µg·L?1, productivity 2.6 0.1 ?g·L?1·h?1. The 1.5 mM L-tyrosine fermentations allowed obtain an 343.3 3.0 48 h, 7.1 This work demonstrates crucial requirement increase 4.3- 10.7-fold.